Glutathione-Agarose Gel H.P.
Also known as: Glutathione-Agarose Gel HP; GST-Agarose Gel HP; GST-Agarose Gel HP, GST-tag Purification Resin
English Name: Glutathione Sepharose H.P.
Product Number: BQ2027
Brand: Bio-Raison
MDL Number: MFCD00131200
Storage: Store at 4°C-25°C (preservation solution is 20% ethanol)
Shelf Life: 1 year
Product Description:
Glutathione-Agarose Gel H.P. is used as an affinity chromatography medium for the separation and purification of glutathione S-transferase (GST)-tagged proteins, other sources of glutathione S-transferases, and proteins with an affinity for glutathione. Proteins expressed using the pGEX vector are fused with glutathione S-transferase, allowing purification through glutathione-agarose affinity chromatography. GSTs are enzymes that use glutathione (γ-glutamylcysteinylglycine) as a substrate to detoxify small toxic molecules by forming thiol esters. Since the affinity of GST for its substrate is in the submillimolar range, GST fusion proteins can be eluted using a buffer containing free glutathione.
Technical Specifications
Product Name | Glutathione-Agarose Gel H.P. |
Matrix | 6% agarose gel |
Ligand | Heparin |
Particle Size | 45-165μm |
Average Particle Size | 34um |
Maximum Flow Rate | 600cm/h |
Working pH Range | 3-12 |
Protein Binding Capacity | 10mg |
Operating Temperature | Room temperature |
保存条件 | Sealed storage at 4-8°C |
Applications:
Affinity chromatography medium for the purification of recombinant fusion proteins containing a GST tag or proteins dependent on S-transferase or glutathione.