Nickel-NTA Agarose Gel 6FF
Aliases: IDA Filler, NTA Filler, His-tag Purification Resin, His-tag; Metal Nickel Chelated Agarose Gel 6FF
English Name: Ni Sepharose 6FF; Ni-NTA Agarose; Ni NTA Agarose; Nickel-NTA Agarose; Ni Sepharose 6 Fast Flow
Product Code: BQ2019
Brand: BioResin
CAS Number: 17948-42-4
Transportation: Strictly prohibited from being mixed or transported with toxic or hazardous materials.
Storage: 2-8°C
Shelf Life: 1 year
Product Overview
Metal chelation affinity chromatography media, also known as immobilized metal ion affinity chromatography (IMAC), operates on the principle of utilizing specific interactions between certain amino acids on the protein surface, such as histidine, and various transition metal ions like Cu²⁺, Zn²⁺, Ni²⁺, Co²⁺, and Fe³⁺. These interactions allow the medium to adsorb proteins rich in these amino acids, achieving separation and purification. As a result, agarose gels conjugated with these metal ions can selectively separate proteins containing multiple histidine residues, as well as peptides, proteins, and nucleotides that interact with metal ions. Generally, Ni²⁺ is the preferred metal ion for purifying histidine-tagged proteins. Cysteine and tryptophan can also bind to immobilized metal ions, but their binding affinity is much weaker than that of histidine residues.
Nickel affinity chromatography media offers advantages such as high specificity and fast flow rates, making it ideal for large-scale production.
Technical Specifications
Product Name | Nickel-NTA Agarose Gel 6FF |
Appearance | Contains 20% ethanol, white gel at the bottom |
Gel Type | Small ligand affinity chromatography filler. |
Composition | 6% cross-linked agarose |
Particle Size | 45-165μm |
Optimal pH Range | 3-12 |
Operating Temperature | 4-30℃ |
Separation Range of Globulins | ~4×10⁶ Dextran (linear molecules): ~2×10⁶ |
Storage Conditions | 4-8℃ |
Applications: Used for the separation of His-tagged recombinant proteins and peptides, proteins, nucleotides, or phosphorylated proteins that can be adsorbed by metal ions.