Product Description:
Ni-Agarose Gel H.P. is a purification medium used for purifying 6×His-tag recombinant proteins. It consists of 6% crosslinked Sepharose coupled with a tetradentate chelator NTA. It can be used to purify 6×His-tag recombinant proteins expressed by any expression system under both native and denaturing conditions. NTA, containing four chelating sites, binds Ni²⁺ more effectively than traditional tridentate chelators. The 6×His-tag binds to Ni²⁺, allowing His-tagged proteins to adhere to the Ni-NTA purification medium. Unbound proteins are washed away, while the bound proteins are gently eluted using a certain concentration of imidazole or low-pH buffer, resulting in high-purity target proteins. This purification medium has an exceptionally high affinity for His-tagged proteins, with a capacity of 5-20 mg/mL. It can purify His-tagged proteins from any expression system under both native and denaturing conditions. The purification process is simple, and the obtained protein purity can reach up to 95%. Ni-NTA can be regenerated and reused 4-6 times. This product is suspended in 20% ethanol and precharged with Ni²⁺.
Technical Specifications
Product Name | Ni-Agarose Gel H.P. |
Appearance | Gel-like, stored in 20% ethanol solution |
Gel Type |
Small ligand affinity chromatography resin |
Composition | 6% crosslinked agarose |
Particle Size | 34μm |
Optimal pH Range | 3-12 |
Operating Temperature | 4-30℃ |
Globulin Separation Range |
|
Storage Conditions | 4℃ |
Applications
Used for the separation of His-tagged recombinant proteins, as well as polypeptides, proteins, nucleotides, and phosphorylated proteins that can be adsorbed by metal ions. Offers high resolution.