Ni-Agarose Gel 6FF
Also known as: Ni-Agarose Gel 6FF; IDA Packing Material; Nickel Column Packing; Nickel Agarose, His-tag Purification Resin, His-tag
English Name: Ni Sepharose 6FF; Ni Sepharose Fast Flow; Ni-Agarose
Product Number: BQ2017
Brand: Bio-Raison
Transport: Must not be mixed or transported with toxic or harmful substances.
Storage: 4°C
Shelf Life: 1 year
Product Description:
Ni NTA Beads 6FF is a purification medium based on highly crosslinked 6% agarose gel. The ligand is the same as that used in Ni NTA Beads, with a protein binding capacity exceeding 40 mg of 6×His-tagged protein per mL of medium. The bead size ranges from 45 to 165 μm. Ni NTA Beads 6FF can withstand harsh reagent conditions (various reducing agents, detergents, high concentrations of denaturing agents) and, due to its pressure-resistant matrix, can tolerate pressures up to 0.3 MPa, making it more stable. This product is ideal for large-scale industrial protein purification at relatively high flow rates. The suspension is 1×PBS with 20% ethanol, pre-charged with Ni²⁺.
技术指标
Product Name |
Ni-Agarose Gel 6FF |
Appearance | Gel-like, stored in 20% ethanol solution |
Gel Type | Small ligand affinity chromatography resin |
Composition | 6% crosslinked agarose |
Particle Size | 45-165μm |
Optimal pH Range | 3-10 |
Operating Temperature | 4-40℃ |
Globulin Separation Range |
|
Storage Conditions | 4℃ |
Applications:
Used for the separation of His-tagged recombinant proteins, as well as polypeptides, proteins, nucleotides, and phosphorylated proteins that can be adsorbed by metal ions.